Madidi's cultural value is equally relevant. Archeological sites of pre-Inca and Inca origin within the protected area and its zone of influence testify to the historical and cultural roots of the people who inhabited this region. Madidi is part of the ancestral territory of the Tacana and Leco peoples. The founding of the Franciscan missionary towns and the arrival of Quechua-speaking groups shaped a new social, cultural and economic reality. The communities currently settled in Madidi are heirs to these cultural traditions and have maintained their governance systems and knowledge of natural resource use.

The cultural importance of Madidi park is increased by the fact that it overlaps with four indigenous territories: San José de Uchupiamonas, Tacana I, Lecos de Apolo and Lecos de Larecaja. Their territorial management experiences contribute to the conservation of biological diversity and generate economic opportunities for the region.

Madidi is one of the most important tourist destinations in Bolivia, benefiting community and municipal initiatives in the region. Experiences in the use of wildlife are producing important results within the framework of territorial management, such as spectacled caiman management, incense and wild cacao harvest, beekeeping, and coffee and native cacao production under agroforestry systems.